Yale University

Yale University
Project #:1U19AI089992-01

Title: Defining Signatures for Immune Responsiveness by Functional Systems Immunology

PI: David A. Hafler (Contact), Erol Fikrig

Yale University

Primary Projects Pilot Projects IOF Projects

The HIPC Center at Yale University employs systems approaches and novel, high throughput and high-fidelity technologies such as multiplexed gene expression, automated multidimensional flow cytometry, and integrated single-cell assays in nanowells to quantitatively assess leukocyte function to ultimately identify the molecular signatures defining individual immune responses. These studies will be done in cohorts of young adults, non-frail elderly, and frail elderly individuals. An influenza vaccine will be used and infection of West Nile virus will be studied.

This functional systems immunology approach will allow us to define baseline human immune signatures following viral infection and vaccination along with deviations from this baseline, with the goal of identifying future targets for intervention and establishing sets of biomarkers that predict responses to vaccination.

Primary Projects

Immune Responses Defining Efficiency of Influenza Vaccination

Project Leader: Albert Shaw

Institution: Yale University

Immune Signatures of Clinical Response to Flavivirus Infections

Project Leader: Erol Fikrig

Institution: Yale University

Mathematical Models for Immune Signatures from Population- and Single-Cell-Based Analyses

Project Leader: Chris Love

Institution: Massachusetts Institute of Technology

Data Repository and Curation Core

Project Leader: Phillip L. De Jager

Institution: Brigham and Women's Hospital Harvard Medical School

Multidimensional Robotic Flow Platform Core

Project Leader: David Hafler

Institution: Yale University

Quantitative Gene Expression Core

Project Leader: Keith Moore

Institution: DHMRI

Immunologic Reagent Resource Core

Project Leader: Vijay Kuchroo

Institution: Brigham and Women's Hospital Harvard Medical School

Pilot Projects

Evaluating Subtype Specific and Cross Reactive T Cell Responses Against the Emerging H1N1 Swine Flu

Project Leader: William Kwok

Institution: Yale University

Evaluating Innate Immune Responses to Staphylococcus Aureus Acquisition and Carriage in Cohorts of Persistent Carriers and Non-Carriers

Project Leader: Barbara Kazmierczak

Institution: Yale University

IOF Projects

Determining the Genetic Basis of Vaccine Response in Humans

Principal Investigator: David Hafler & Chris Costapas

Institution: Yale University

High-Throughput Immunophenotypic Analyses of Humoral Responses to West Nile Virus

Principal Investigator: John Christopher Love

Institution: Massachusetts Institute of Technology

Development of HIPC Data Standards to Support Cross-Center Projects

Principal Investigator: Steven Kleinstein

Institution: Yale University

Deciphering the Role of Natural Killer Cells in Resistance to Infection with West Nile Virus

Principal Investigator: Ruth Montgomery

Institution: Yale University

Effects of Aging on STAT3 Function in T cells

Principal Investigator: Susan Kaech

Institution: Yale University

Integration of HIPC Data to Identify Common Signatures of Influenza Vaccination Responses

Principal Investigator: Steven Kleinstein

Institution: Yale University

U.S.-India Bilateral Collaborative Research Projects

Immune Signatures of Responses to Infection with Dengue Virus

HIPC Project Leaders: Ruth Montgomery and Priti Kumar

HIPC Institution: Yale University

Indian Project Leader: V. Ravi

Indian Institution: National Institute of Mental Health/Neurosciences, India