Stanford University

Stanford University
Project #:1U19AI090019-01

Title: Vaccination and Infection: Indicators of Immunological Health and Responsiveness

PI: Mark M. Davis

Website: iti.stanford.edu

Stanford University

Primary Projects Pilot Projects IOF Projects

The HIPC Center at Stanford University aims to use a systems biology approach, such as high throughput HLA sequencing technique, selected Immunoglobulin and T Cell Receptor repertoire analysis, combinatorial peptide-MHC tetramer analysis, and advanced bioinformatics analysis such as our new cell type specific SAM algorithms (csSAM), to survey immune responsiveness across a range of different vaccines, including influenza, both seasonal and the new pandemic H1N1v, Varicella zoster, and the bacterium Neisseria meningitidis. These investigations will compare different age groups-children, young adults, and the elderly-to look for specific markers and assays of immune competence and especially those that might be common across different pathogens and age groups. We will also survey the influenza vaccine responses of individuals with impaired or dysregulated immune systems in order to see how these might deviate from healthy individuals, and assess the natural immune responses to influenza and Varicella zoster infection.

Analysis of a variety of different disease/vaccine models will aid in defining common and unique characteristics of responder and non-responder individuals to develop new informative tools and assays that should be illuminating both to specific questions regarding these study groups and may also be of benefit generally with respect to "metrics" of immunological health and dysfunction.

Primary Projects

Plasmablast Trafficking and Antibody Response in Influenza Infection and Vaccination

Project Leader: Harry Greenberg

Institution: Stanford University

T Cell Responses To H1N1v and a Longitudinal Study of Seasonal Flu Vaccination

Project Leader: Mark Davis

Institution: Stanford University

Measuring Influenza and H1N1 Vaccine Responses in Immunodeficient Patients

Project Leader: Paul J. Utz

Institution: Stanford University

T Cell Responses to Varicella Zoster Virus After Vaccination and Viral Escape

Project Leader: Jorg J. Goronzy

Institution: VA Palo Alto Health Care System

Analysis of immune receptor diversity and oligoclonality in responses to vaccination

Project Leader: Andrew Fire

Institution: Stanford University

New Computational Methods for Analyzing the Response to Vaccination and Infection

Project Leader: Daphne Koller

Institution: Stanford University

Human Immune Monitoring Core

Project Leader: Holden Maecker

Institution: Stanford University

Genomics Core

Project Leader: Michael Mindrinos

Institution: Stanford University

Clinical Core

Project Leader: Cornelia Dekker

Institution: Stanford University

Biostatistics Core

Project Leader: Cornelia Dekker

Institution: Stanford University

Pilot Projects

Role of Dendritic Cell Subsets in the Immune Response to Vaccination

Project Leader: Edgar Engleman

Institution: Stanford University

Temporal Analysis of TCR Signaling in Young and Old Individuals using a Novel Microfluidic Phosphoflow Platform

Project Leader: Gary P. Nolan

Institution: Stanford University

Gene Expression Signature of Protection and Response to Seasonal Influenza (TIV) Vaccine

Project Leader: Michael P. Snyder

Institution: Stanford University

Lithographic Peptide Arrays to Monitor Flu Responses

Project Leader: Paul J. Utz

Institution: Stanford University

IOF Projects

Shared Resource for Mass Cytometry (CyTOF)

Principal Investigator Holden Maecker

Institution: Stanford University

The Influence of Chronic Cytomegalovirus Infection on Influenza Vaccine Responses

Principal Investigator Jorg Goronzy

Institution: Stanford University

The Human Mucosal Immune Responses to Influenza Virus: A Systems Biology Approach

Principal Investigator Harry Greenberg

Institution: Stanford University

Enabling Advanced High and Low level Analytics for Luminex Technology

Principal Investigator Ofir Goldberger

Institution: Stanford University

Deconvolution of High Bandwidth Data to Yield Cell-Centered View of Immunity

Principal Investigator Shai Shen-Orr

Institution: Technion

Immune Signatures of Human Pregnancy

Principal Investigator Catherine Blish

Institution: Stanford University

Clonal Analysis of Tissue Specific Immune Responses in Man

Principal Investigator Eugene Butcher

Institution: Stanford University

Tools for Deep Phenotypic and Functional Analysis of Healthy Human Basophil and Eosinophil Granulocytes

Principal Investigator Stephen Galli

Institution: Stanford University

The Adaptive Immune Response to an Influenza Vaccine with a Unique, Non-Inflammatory Adjuvant

Principal Investigator Mark Davis

Institution: Stanford University

Non-Invasive Mass Cytometric Monitoring of Tissue Specific Immune Responses in Man

Principal Investigator Eugene Butcher

Institution: Stanford University

Influenza Tetramers and CyTOF

Principal Investigator Holden Maecker

Institution: Stanford University

U.S.-India Bilateral Collaborative Research Projects

B and T Cell Responses to Rotavirus Infection and Vaccination in Indian Infants

HIPC Project Leader: Harry Greenberg

HIPC Institution: Stanford University

Indian Project Leader: Gagandeep Kang

Indian Institution: Christian Medical College

Neonatal Immune Profiles: Infections and Toxicants

HIPC Project Leader: Holden Maecker

HIPC Institution: Stanford University

Indian Project Leader: Satyajit Rath

Indian Institution: National Institute of Immunology