Seattle Biomedical Research Institute

Seattle Biomedical Research Institute
Project #:1U19AI089986-01

Title: Immune Profile and Network Analysis of Malaria Infection and Vaccination

PI: Kenneth D. Stuart (Contact), Margaret Juliana McElrath, Ruobing Wang

Seattle Biomedical Research Institute

Primary Projects IOF Projects

The HIPC Center at Seattle Biomedical Research Institute focuses on the characterization of antimalarial immune responses and their relationship to immune protection. The project will use a wide range of best-of-breed methodologies to characterize a wide range of immune responses and immune cell types. This includes multi-parametric assessments of malaria specific leukocyte phenotypes, cytokine and chemokine profiles, antibody responses, and transcriptional and host DNA analyses. Samples will be obtained prior to and following vaccination and challenge or natural infection from study populations that span malaria-naive adults to children and adults living in endemic regions. The P. falciparum parasites range from well characterized clinical isolates (e.g. NF54) to biodiverse natural populations in several African countries.

The study is designed to provide insight into protective immune mechanisms to malaria in order to improve anti-malarial vaccines and also to lay a foundation for improved vaccine development in general that can be shared with the community.

Primary Projects

Immune Signatures of Protection Induced by Whole Parasite Malaria Vaccines

Project Leader: Ruobing Wang

Institution: Seattle Biomedical Research Institute

Immune Signatures Associated with Plasmodium falciparum Control Following RTS.S/ASO1E Vaccination and Natural Infection

Project Leader: M. Juliana McElrath

Institution: Fred Hutchinson Cancer Research Center

IOF Projects

Standardization of Flow Cytometry Data Analysis Using Lyoplates and FITMaN Marker Panels

Principal Investigator: Raphael Gottardo

Institution: Fred Hutchinson Cancer Research Center

Benchmarking and Standardizing High Dimensional Single Cell Data Analysis Methods

Principal Investigator: Raphael Gottardo

Institution: Fred Hutchinson Cancer Research Center

Quality Versus Quantity: Comparison of Antibody Concentration and Functionality in Different Malaria Vaccine Strategies

Principal Investigator: Ruobing Wang

Institution: Seattle Biomedical Research Institute

Enabling Advanced High and Low Level Analytics for Luminex Technology

Principal Investigator: Raphael Gottardo

Institution: Fred Hutchinson Cancer Research Center

Unbiased Functional Profiling of the Mucosal-Associated Invariant T cell Response Following Malaria Infection

Principal Investigator: Martin Prlic

Institution: Fred Hutchinson Cancer Research Center

U.S.-India Bilateral Collaborative Research Projects

Innate Predictors of Adaptive BCG and HBV Vaccine-Induced Responses in Bangalore Adolescents

HIPC Project Leader: Juliana McElrath

HIPC Institution: Seattle Biomedical Research Institute

Indian Project Leader: Annapurna Vyakarnam

Indian Institution: India Institute of Science