Icahn School of Medicine at Mount Sinai/ University of California, Berkeley
Title: Dengue Human Immunology Project Consortium (DHIPC)
PI: Ana Fernandez-Sesma and Eva Harris
The Dengue Human Immunology Project Consortium (DHIPC) has established a comprehensive program for assessing the dynamic human immune response to dengue virus (DENV) infection and vaccination with a holistic approach using a compendium of cutting-edge systems biology techniques. The DHIPC will apply this approach to a) well-characterized clinical samples derived from children from three distinct long-term dengue studies in Nicaragua, b) samples from healthy men and women enrolled in clinical vaccine trials and human challenge studies, and c) robust ex vivo human systems for DENV infection. Bioinformatics analyses and modeling across data sets will lead to the identification of mechanisms underlying the human immune response to infection or vaccination against dengue, as well as of genetic factors contributing to outcome.
Dengue is the most prevalent mosquito-borne viral disease of humans. The studies conducted by the DHIPC will contribute to the identification of immune and genetic signatures that can be used as predictors or indicators of protection or disease severity and will represent a new paradigm for studying other human infectious diseases.
Primary Projects
Immune profiling of natural dengue virus infections
Project Leader: Eva Harris
Institution: University of California, Berkeley
Immune profiling of candidate vaccines for dengue
Project Leader: Jorge E. Osorio
Institution: University of Wisconsin, Madison
Ex vivo immune profiling of dengue viruses and vaccines
Project Leader: Ana Fernandez-Sesma
Institution: Icahn School of Medicine at Mount Sinai
Genomics Core
Project Leader: Steven M. Wolinsky
Institution: Northwestern University
Proteomics Core
Project Leader: Nevan J. Krogan
Institution: The J. David Gladstone Institutes
Immune Monitoring Core
Project Leader: Miriam Merad
Institution: Icahn School of Medicine at Mount Sinai
Data analysis and Modeling Core
Project Leader: Eric E. Schadt
Institution: Icahn School of Medicine at Mount Sinai
Data management and Dissemination Core
Project Leader: Andrew Kaasarskis
Institution: Icahn School of Medicine at Mount Sinai
IOF Management Core
Project Leader: Adolfo Garcia-Sastre
Institution: Icahn School of Medicine at Mount Sinai
IOF Projects
HIPC Web Portal
Project Leader: Charlie Quinn
Institution: Benaroya Research Institute
Development of a High-throughput Pipeline for Host Gene Knockout in Dengue Primary Cell Types
Project Leader: Nevan Krogan
Institution: The J. David Gladstone Institutes
Immune profiling and network modeling of Chikungunya infections
Project Leader: Eva Harris
Institution: The Regents of the University of California, Berkeley
Development of the HIPC Database and Research Portal
Project Leader: Raphael Gottardo
Institution: Fred Hutchinson Cancer Research Center
ImmuneRegulation: An ImmuneSpace module for identifying human immune regulatory elements
Project Leader: Zeynep Gumus
Institution: Icahn School of Medicine at Mount Sinai
Ontology Standards Consultation
Project Leader: Adeeb Rahman
Institution: Icahn School of Medicine at Mount Sinai
Development and optimization of lyophilized reagents to improve data quality, reproducibility and meta-analyses across CyTOF studies
Project Leader: Adeeb Rahman
Institution: Icahn School of Medicine at Mt. Sinai
Zika Supplements
Profiling and network modeling of innate immunity to human Zika virus infection in Dengue negative and Dengue positive children from long-standing Nicaraguan cohorts
Project Leader: Eva Harris
Institution: University of California, Berkeley
Developing the Oxford Nanopore MinION system for real-time cDNA/RNA sequencing of Dengue, Chikungunya, and Zika viruses
Project Leader: Steven Wolinsky
Institution: Icahn School of Medicine at Mount Sinai
Developing an ex vivo system using primary human cells infected with relevant ZIKV isolates to study the tropism of the virus in human immune cells and the immune activation profile using mass cytometry (CyTOF), luminex technology and proteomics approaches
Project Leader: Irene Ramos
Institution: Icahn School of Medicine at Mount Sinai
Immunologic analysis of maternal samples from the NIAID/NICHD Zika in Infants and Pregnancy (ZIP) program
Project Leader 1: Eva Harris
Institution: University of California, Berkeley
Project Leader 2: Ruth Montgomery
Institution: Yale University
Immune Variation from Infancy to Adolescence: Dissecting the Effects of Age, Gender, And Individuality (ZIKA ZIP Study and HIPC Center Collaboration)
Project Leader 1: Eva Harris
Institution: University of California, Berkeley
Project Leader 2: Ana Fernandez-Sesma
Institution: Icahn School of Medicine at Mount Sinai
Universal Influenza Vaccine Supplements
Systems Immunology of Human Tonsil Cultures to Profile Innate and Adaptive Immunity to Influenza Infection and Various Candidate Influenza Vaccines
Project Leader 1: Ana Fernandez-Sesma
Institution: Icahn School of Medicine at Mount Sinai
Project Leader 2: Eva Harris
Institution: University of California, Berkeley