Icahn School of Medicine at Mount Sinai/ University of California, Berkeley

Project #:1-U19-AI-118610-01

Title: Dengue Human Immunology Project Consortium (DHIPC)

PI: Ana Fernandez-Sesma and Eva Harris

MSSM

Primary Projects IOF Projects

The Dengue Human Immunology Project Consortium (DHIPC) has established a comprehensive program for assessing the dynamic human immune response to dengue virus (DENV) infection and vaccination with a holistic approach using a compendium of cutting-edge systems biology techniques. The DHIPC will apply this approach to a) well-characterized clinical samples derived from children from three distinct long-term dengue studies in Nicaragua, b) samples from healthy men and women enrolled in clinical vaccine trials and human challenge studies, and c) robust ex vivo human systems for DENV infection. Bioinformatics analyses and modeling across data sets will lead to the identification of mechanisms underlying the human immune response to infection or vaccination against dengue, as well as of genetic factors contributing to outcome.

Dengue is the most prevalent mosquito-borne viral disease of humans. The studies conducted by the DHIPC will contribute to the identification of immune and genetic signatures that can be used as predictors or indicators of protection or disease severity and will represent a new paradigm for studying other human infectious diseases.

Primary Projects

Immune profiling of natural dengue virus infections

Project Leader: Eva Harris

Institution: University of California, Berkeley

Immune profiling of candidate vaccines for dengue

Project Leader: Jorge E. Osorio

Institution: University of Wisconsin, Madison

Ex vivo immune profiling of dengue viruses and vaccines

Project Leader: Ana Fernandez-Sesma

Institution: Icahn School of Medicine at Mount Sinai

Genomics Core

Project Leader: Steven M. Wolinsky

Institution: Northwestern University

Proteomics Core

Project Leader: Nevan J. Krogan

Institution: The J. David Gladstone Institutes

Immune Monitoring Core

Project Leader: Miriam Merad

Institution: Icahn School of Medicine at Mount Sinai

Data analysis and Modeling Core

Project Leader: Eric E. Schadt

Institution: Icahn School of Medicine at Mount Sinai

Data management and Dissemination Core

Project Leader: Andrew Kaasarskis

Institution: Icahn School of Medicine at Mount Sinai

IOF Management Core

Project Leader: Adolfo Garcia-Sastre

Institution: Icahn School of Medicine at Mount Sinai

IOF Projects

HIPC Web Portal

Project Leader: Charlie Quinn

Institution: Benaroya Research Institute

Development of a High-throughput Pipeline for Host Gene Knockout in Dengue Primary Cell Types

Project Leader: Nevan Krogan

Institution: The J. David Gladstone Institutes

Immune profiling and network modeling of Chikungunya infections

Project Leader: Eva Harris

Institution: The Regents of the University of California, Berkeley