La Jolla Institute for Allergy and Immunology
Title: Human immune signatures of Dengue virus and Mycobacterium Tuberculosis exposure in infection, disease and vaccination
PI: Alessandro D. Sette
The HIPC center at the La Jolla Institute is elucidating T cell signatures of protection from and susceptibility to disease in an unbiased manner. To gain a broad understanding of T cell responses, both a bacterial (tuberculosis) and a viral (dengue) disease will be studied, using samples from geographically and ethnically diverse cohorts. These pathogens were also chosen for their global health significance; this work has high potential to inform the development and testing of improved vaccines, as T cells are known to be key for protective immunity against both tuberculosis and dengue. The two projects have parallel designs, defining and comparing various T cell subsets from cohorts with past or latent infection, active disease, and vaccination (with an experimental dengue vaccine or the yellow fever vaccine for the dengue studies and with the BCG vaccine for tuberculosis).
The fundamental impact of this research will be greater insight into whether infection and vaccination lead to expansion or contraction of subsets already present within the individual’s T cell pool that maintain their phenotype, and/or lead to differentiation or activation of T cells into states uniquely associated with those conditions.
Primary Projects
Human immune signatures of natural immunity to DENV infection, severe disease, and vaccination with attenuated live vaccine
Project Leader: Alessandro D. Sette
Institution: La Jolla Institute for Allergy and Immunology
Human immune signatures of latent MTB infection, active disease and BCG vaccination
Project Leader: Bjoern Peters
Institution: La Jolla Institute for Allergy and Immunology
Epigenomics of DENV and MTB
Project Leader: Pandurangan Vijayanand
Institution: La Jolla Institute for Allergy and Immunology
Sequencing Core
Project Leader: Gregory Seumois
Institution: La Jolla Institute for Allergy and Immunology
Clinical Core
Project Leader: Pandurangan Vijayanand
Institution: La Jolla Institute for Allergy and Immunology
Data Management Core
Project Leader: Bjoern Peters
Institution: La Jolla Institute for Allergy and Immunology
IOF Projects
Shared resource for micro-scaled epigenetic assays
Project Leader: Pandurangan Vijayanand
Institution: La Jolla Institute for Allergy and Immunology
Shared resource for functional genomics
Project Leader: Sonia Sharma
Institution: La Jolla Institute for Allergy and Immunology
Phenotyping and mRNA profiling of pertussis-specific CD4 T cells induced by acellular versus whole-cell vaccines associated with differential protective efficacy
Project Leader: Alessandro D. Sette
Institution: La Jolla Institute for Allergy and Immunology
Single-cell RNA-Seq analysis of MTB and DENV immune responses
Project Leader: Pandurangan Vijayanand
Institution: La Jolla Institute for Allergy and Immunology
Development of HIPC data standards
Project Leader: Bjoern Peters
Institution: La Jolla Institute for Allergy and Immunology
Phenotyping and mRNA profiling or pertussis-specific CD4 T cells induced by acellular versus whole-cell vaccines associated with differential protective efficacy
Project Leader: Alessandro Sette
Institution: La Jolla Institute for Allergy and Immunology
Expansion of the FlowGate infrastructure to support the analysis and integration of the flow and mass cytometry data
Project Leader: Richard Scheuermann
Institution: J. Craig Venter Institute
Continued and expanded computational analysis and integration of flow and mass cytometry data across HIPC centers
Project Leader: Richard Scheuermann
Institution: J. Craig Venter Institute
Cellular and transcriptional immunosignatures to predict development of plasma leakage in acute Dengue infection
Project Leader: Daniela Weiskopf
Institution: La Jolla Institute for Allergy and Immunology
HIPC SUPPLEMENT: Assessing the Immune Signature of Monocytes that Correlates with Protection from Active Tuberculosis Infection
Project Leader: Alessandro D. Sette
Institution: La Jolla Institute for Allergy and Immunology
HIPC SUPPLEMENT: Identifying HLA Alleles Associated with Protection During MTB Infection
Project Leader: Alessandro D. Sette
Institution: La Jolla Institute for Allergy and Immunology
HIPC SUPPLEMENT: Immune-Based Signature Analysis of Shringrix Vacinnees
Project Leader: Alessandro D. Sette
Institution: La Jolla Institute for Allergy and Immunology
Zika Supplements
Characterizing the CD4 and CD8 T cell response to Zika: phenotyping and mRNA profiling of effector and memory T cells from Zika infected and uninfected individuals
Project Leader: Daniela Weiskopf
Institution: La Jolla Institute for Allergy and Immunology
Universal Influenza Vaccine Supplements
Using single cell RNASeq to analyze specific immune cell correlates in response to an adjuvanted influenza vaccination
Project Leader: Alessandro D. Sette
Institution: La Jolla Institute for Allergy and Immunology
Indo-U.S. Vaccine Action Program Projects
Comprehensive Analysis of the Follicular Helper T Cell Repertoire in Flavivirus Infection and Vaccination
HIPC Project Leader: Shane Crotty
Institution: La Jolla Institute for Allergy and Immunology
Indian Project Leader: Nimesh Gupta
Institution: National Institute of Immunology