Dana-Farber Cancer Institute

Dana-Farber Cancer Institute
Project #:1U01AI090043-01

Title: Crossprotective CTL Against Influenza

PI: Ellis L. Reinherz

Dana-Farber Cancer Institute

Primary Projects Pilot Projects IOF Projects

The HIPC Center at Dana-Farber uses recent advances in bioinformatics and proteomics, such as cDNA microarray for rapid HLA profiling and our novel ultrasensitive MS3 method for peptidome analysis to explore the potential to elicit protective CD8 T cell responses against 153 conserved epitopes derived from 5,315 sets of influenza A taxonomy data covering all internal and surface viral proteins and predicted to bind to common HLA alleles such as HLA-A*0201. These HLA restricted epitopes are capable of affording broad population protection coverage as determined through recently developed bioinformatics methodologies. To do these studies H1N1, H2N2 and H3N2 influenza A infected human epithelial cells, and memory T cells from naturally exposed adults or T cells from LAIV-vaccinated children and adults will be tested.

This information has the potential to provide the basis, in the near-term, for development of a universal influenza A vaccine, affording protection against seasonal variants as well as pandemic strains of influenza A viruses. It has general application to other highly mutable viral pathogens affecting mankind.

Primary Projects

Crossprotective CTL Against Influenza

Project Leader: Ellis Reinherz

Institution: Dana-Farber Cancer Institute

Pilot Projects

Human KIR Variation Profiling by Microarray

Project Leader: Vladimir Brusic

Institution: Dana-Farber Cancer Institute

IOF Projects

Comparison and Standardization of Advanced HLA Typing Methods

Project Leader: Vladimir Brusic

Institution: Dana-Farber Cancer Institute

Bioinformatics Infrastructure for HIPC: Planning and Initiation Phase

Project Leader: Vladimir Brusic

Institution: Dana-Farber Cancer Institute

Nanoscale MSE Poisson Detection of MHC I Peptides

Project Leader: Bruce Reinhold

Institution: Dana-Farber Cancer Institute

Identification of Early Innate, B Cell, and CD4+ T Follicular Helper Cell Responses After Dengue Vaccination which Predict a High Titer Cross-Neutralizing Antibody Response

Project Leader: Sarah George

Institution: Saint Louis University Vaccine Testing and Evaluation Unit (VTEU)

Bioinformatics Infrastructure for HIPC: Design and Oversight Phase

Project Leader: Vladimir Brusic

Institution: Dana-Farber Cancer Institute

Bioinformatics Infrastructure for HIPC: Coordination of Implementation Phase

Project Leader: Vladimir Brusic

Institution: Dana-Farber Cancer Institute