Yale University
Title: Defining Signatures for Immune Responsiveness by Functional Systems Immunology
PI: David A. Hafler (Contact), Erol Fikrig
Primary Projects Pilot Projects IOF Projects
The HIPC Center at Yale University employs systems approaches and novel, high throughput and high-fidelity technologies such as multiplexed gene expression, automated multidimensional flow cytometry, and integrated single-cell assays in nanowells to quantitatively assess leukocyte function to ultimately identify the molecular signatures defining individual immune responses. These studies will be done in cohorts of young adults, non-frail elderly, and frail elderly individuals. An influenza vaccine will be used and infection of West Nile virus will be studied.
This functional systems immunology approach will allow us to define baseline human immune signatures following viral infection and vaccination along with deviations from this baseline, with the goal of identifying future targets for intervention and establishing sets of biomarkers that predict responses to vaccination.
Primary Projects
Immune Responses Defining Efficiency of Influenza Vaccination
Project Leader: Albert Shaw
Institution: Yale University
Immune Signatures of Clinical Response to Flavivirus Infections
Project Leader: Erol Fikrig
Institution: Yale University
Mathematical Models for Immune Signatures from Population- and Single-Cell-Based Analyses
Project Leader: Chris Love
Institution: Massachusetts Institute of Technology
Data Repository and Curation Core
Project Leader: Phillip L. De Jager
Institution: Brigham and Women's Hospital Harvard Medical School
Multidimensional Robotic Flow Platform Core
Project Leader: David Hafler
Institution: Yale University
Quantitative Gene Expression Core
Project Leader: Keith Moore
Institution: DHMRI
Immunologic Reagent Resource Core
Project Leader: Vijay Kuchroo
Institution: Brigham and Women's Hospital Harvard Medical School
Pilot Projects
Evaluating Subtype Specific and Cross Reactive T Cell Responses Against the Emerging H1N1 Swine Flu
Project Leader: William Kwok
Institution: Yale University
Evaluating Innate Immune Responses to Staphylococcus Aureus Acquisition and Carriage in Cohorts of Persistent Carriers and Non-Carriers
Project Leader: Barbara Kazmierczak
Institution: Yale University
IOF Projects
Determining the Genetic Basis of Vaccine Response in Humans
Principal Investigator: David Hafler & Chris Costapas
Institution: Yale University
High-Throughput Immunophenotypic Analyses of Humoral Responses to West Nile Virus
Principal Investigator: John Christopher Love
Institution: Massachusetts Institute of Technology
Development of HIPC Data Standards to Support Cross-Center Projects
Principal Investigator: Steven Kleinstein
Institution: Yale University